Directed Research Program

NIH City College of San Francisco/San Francisco State University
Bridges to Baccalaureate Program
Directed Research Program
Summer 2009

Instructors: Sally Pasion, SFSU & Chantilly Munson, CCSF

JUNE 8 - JULY 1, 2009
Instructor: Sally Pasion, Ph.D. Teaching Assistant: Terry Reyes
click on week below to view class agendas and download course materials

WEEK 1 Jun 8- Jun 12, 2009

WEEK 2 Jun 15 - Jun 19, 2009

WEEK 3 Jun 22 - Jun 26, 2009

WEEK 4.5 Jun 29 - Jul 1, 2009

click on this link to view course materials for Weeks 4.5 - 7 (Jul 6-Jul 28, 2009)

WEEK 1 June 8- June 12, 2009

Monday 6/08 Tuesday 6/09 Wednesday 6/10 Thursday 6/11 Friday 6/12

Monday, June 8, 2009: Orientation Day back to top of page

9:00 am - 10:30 am	Introductions
10:30 am - 11:00 am	Keeping a Lab Notebook
11:00 am - 12 noon	Searching for articles
	Task 1 web site: http://www.nlm.nih.gov/bsd/disted/pubmed.html
	Cell and Molecular and Microbiology Online journals available by SFSU subscription
12 noon - 1:00 pm	lunch break
1:30 pm - 4:00 pm	Lab Safety
1 pm - 2 pm	Lab Notebooks
2 pm - 5 pm	Task 3 web site 1: http://www.fireextinguisher.com/
2 pm - 5 pm	Task 3 web site 2: http://ehs.okstate.edu/modules/index.htm?option=com_extcalendar&Itemid=59&extmode=view&extid=287
	Task 3 web site 3: http://ehs.uky.edu/classes/fumehood/train.html
	Task 4 web site 1: http://www.free-training.com/osha/hazcom/hazmenu.htm
	Task 4 web site 2: http://hazard.com/msds/index.php
	Task 5: Scavenger Hunt
	Bioethics session: Case studies reviewed in 5 breakout groups (handouts in binder)

Additional sites of interest:
Guide to Gloves http://www.hazmat.msu.edu:591/glove_guide/

Tuesday 6/09/09 back to top of page

9:30 am - 11:00 am	Introduction to fission yeast
11:00 am - 12 noon	Exercise 1-1: Genotype & phenotype
12 noon - 1:00 pm	lunch break
1:00 pm - 2:00 pm	Exercise 2: Pipetman practice
2:00 pm - 3:30 pm	Introduction to bacteria and plasmids
3:30 pm - 4:00 pm	Microbiology sterile technique demo
4:00 pm - 4:30 pm	Exercise 3-1: Plasmid DNA isolation
4:30 pm - 5:00 pm	Lab clean-up

Wednesday 6/10/09 back to top of page

9:30 am - 10:30 am	Exercise 4: How to use microscope
10:30 am - 11:00 am	Fission Yeast Morphology
11:00 am - 12 noon	Exercise 5: Fission Yeast microscope observation
12 noon - 1:00 pm	lunch break
1:00 pm - 2:00 pm	Continue Exercise 5
2:00 pm - 4:30 pm	Exercise 3-2: Plasmid DNA isolation
4:30 pm - 5:00 pm	Lab clean-up

Thursday 6/11/09 back to top of page

9:30 am - 10:30 am	DNA replication DNA replication overview movie with narration: http://www.bio.cmu.edu/courses/03121/TechTeach/replication/DNARepOverview.mov Lagging Strand DNA replication movie: http://www.bio.cmu.edu/courses/03121/TechTeach/replication/RepOkaz.html DNA Replication and Central Dogma animations: http://www.wehi.edu.au/wehi-tv/dna/index.html DNA replication animation with narration: http://www.youtube.com/watch?v=4jtmOZaIvS0
10:30 am - 12:00 noon	UV and DNA damage Thymine dimer formation and repair: http://highered.mcgraw-hill.com/olc/dl/120082/micro18.swf
12 noon - 1:00 pm	lunch break
1:00 pm - 3:00 pm	Exercise 6-1: Yeast UV sensitivity [Word document Exercise06-1.doc]
3:00 pm - 3:30 pm	Restriction Enzyme Digestions
3:30 pm - 4:30 pm	Exercise 7-1: Restriction enzyme digestion
4:30 pm - 5:00 pm	Lab Clean-up

Friday 6/12/09 back to top of page

9:30 am - 10:00 am	Gel electrophoresis Gel electrophoresis animation http://learn.genetics.utah.edu/units/biotech/gel/
10:00 am - 12 noon	Exercise 1-2: Genotype & phenotype
10:00 am - 12 noon	Exercise 7-2: Restriction Enzyme Digestion
12 noon - 1:00 pm	lunch break
1:00 pm - 2:30 pm	MOVIE
2:30 pm - 4:00 pm	Continue Exercise 1-2
2:30 pm - 4:00 pm	Continue Exercise 7-2
4:00 pm - 4:30 pm	Discussion
4:30 pm - 5:00 pm	Lab clean-up

WEEK 2 June 15 - June 19, 2009 back to top of page

Monday 6/15 Tuesday 6/16 Wednesday 6/17 Thursday 6/18 Friday 6/19

Monday 6/15/09 back to top of page

9:30 am - 10:00 am	Exercise 1-3: Genotype & Phenotype
10:00 am - 12:00 pm	Exercise 6-2: Yeast UV sensitivity UV Viability Summary (pdf)
12:00 pm - 1:00 pm	lunch break
1:00 pm - 2:00 pm	Yeast transformation
2:00 pm - 3:30 pm	Exercise 8-1: Yeast transformation
3:30 pm - 4:30 pm	Continue Exercises 1-3 and 6-2
4:30 pm - 5:00 pm	Lab Clean-up

NOTE: Lab notebooks will be picked up at the end of the day

Tuesday 6/16/09 back to top of page

9:30 am - 11:00 am	Exercise 9-1: Media preparation
10:30 am - 12:00 noon	Exercise 10: What is your gene? [Word document Exercise10.doc]]
12:00 pm - 1:00 pm	lunch break
1:00 pm - 2:30 pm	Continue Exercise 10
1:00 pm - 2:30 pm	Continue Exercise 9-1
2:30 pm - 3:30 pm	Report to Class
3:30 pm - 5:00 pm	Exercise 11-1: Independent UV experiment
4:30 pm - 5:00 pm	Lab Clean-up

Wednesday 6/17/09 back to top of page

9:30 am - 11:00 am	Continue Exercise 11-1
11:00 am - 12:00 pm	Report to Class
12:00 pm - 1:00 pm	lunch break
1:00 pm - 2:30 pm	MOVIE
2:30 pm - 5:00 pm	Exercise 11-2: Independent UV experiment
4:30 pm - 5 pm	Lab Clean-up

Thursday 6/18/09 back to top of page

9:30 am - 10:00 am	Exercise 12: Yeast Growth Curve
11:00 am - 12:00 pm	Continue Exercise 11-2
12:00 pm - 1:00 pm	lunch break
1:00 pm - 2:30 pm	Report to class Exercise 11-2 and discussion
1:00 pm - 4:30 pm	Continue Exercise 12-1
3:30 pm - 5:00 pm	Exercise 11-1: Independent UV experiment
	•e-mail Exercise 11-3 protocol by 4pm •turn in Exercise 11-3 Prep worksheet
4:30 pm - 5:00 pm	Lab Clean-up

Friday 6/19/09 back to top of page

Solar UV 6/19/09 pdf

9:30 am - 12 noon	Exercise 11-3: Independent UV experiment
12:00 pm - 1:00 pm	lunch break
1:00 pm - 4:30 pm	Exercise 11-3: Independent UV experiment
3:30 pm - 4:00 pm	Meiosis presentation
4:00 pm - 4:30 pm	Exercise 13-1: Mating Type Check
4:30 pm - 5:00 pm	Lab Clean-up

WEEK 3 June 22 - June 26, 2009 back to top of page
Monday 6/22 Tuesday 6/23 Wednesday 6/24 Thursday 6/25 Friday 6/26

Monday 6/22/09 back to top of page

9:30 am - 12:00 noon	Exercise 8-2: Yeast transformation
9:30 am - 12:00 noon	Exercise 9-2: Media Preparation
12:00 pm - 1:00 pm	lunch break
1:00 pm - 4:30 pm	Continue Exercise 8-2
1:00 pm - 4:30 pm	Continue Exercise 9-2
4:30 pm - 5:00 pm	Lab Clean-up

Tuesday 6/23/09 back to top of page

9:30 am - 12:00 noon	Exercise 11-4: Independent UV Experiment
12:00 pm - 1:00 pm	lunch break
1:00 pm - 4:00 pm	Exercise 13-2: Mating type check
1:00 pm - 4:00 pm	Continue Exercise 11-4
4:00 pm - 4:30 pm	Report results for Exercise 13-2
4:30 pm - 5:00 pm	Lab Clean-up

Wednesday 6/24/09 back to top of page

9:30 am - 12:00 noon	Discussion about Poster preparation Poster presentation tutorial web site: http://www.kumc.edu/SAH/OTEd/jradel/Poster_Presentations/PstrStart.html
10:30 am - 12:00 noon	Prepare Exercise 11-5 protocol
12:00 pm - 1:00 pm	lunch break
1:00 pm - 4:30 pm	Exercise 14-1: Suppressor screen
3:00 pm - 4:30 pm	Prepare Exercise 11-5 protocol
	•E-mail to instructor Exercise 11-5 protocol by 4pm •turn in exercise 11-5 Prep worksheet
4:30 pm - 5:00 pm	Lab Clean-up

Thursday 6/25/09 back to top of page

Solar UV 6/25/09 pdf

9:30 am - 12:00 noon	Exercise 11-5: Independent UV experiment
9:30 am - 12:00 noon	Research time for poster prep
12:00 pm - 1:00 pm	lunch break
1:00 pm - 4:30 pm	Exercise 11-5: Independent UV experiment
1:00 pm - 4:30 pm	Research time for poster prep
4:30 pm - 5:00 pm	Lab Clean-up

Friday 6/26/09 back to top of page

9:30 am - 11:00 am	Exercise 8-3: Yeast transformation
11:00 am - 12:00 noon	Report results for Exercise 8-3
12:00 pm - 1:00 pm	lunch break
1:00 pm - 2:30 pm	DOCUMENTARY
2:30 pm - 3:30 pm	Discussion
3:30 pm - 4:30 pm	Research time for poster prep
4:30 pm - 5:00 pm	Lab Clean-up

WEEK 4 June 29 - July 1, 2009 back to top of page
Monday 6/29 Tuesday 6/30 Wednesday 7/01

Monday 6/29/09 back to top of page

9:30 am - 12:00 noon	Exercise 11-6: Independent UV experiment
12:00 pm - 1:00 pm	lunch break
1:00 pm - 4:30 pm	Poster Prep
4:30 pm - 5:00 pm	Lab Clean-up

Tuesday 6/30/09 back to top of page

9:30 am - 12:00 noon	Poster Prep
12:00 pm - 1:00 pm	lunch break
1:00 pm - 4:30 pm	Poster Prep
4:30 pm - 5:00 pm	Lab Clean-up

Wednesday 7/01/09 back to top of page

9:30 am - 12:00 noon	Poster Prep
12:00 pm - 1:00 pm	lunch break
1:00 pm - 4:30 pm	Exercise 14-2: Suppressor Screen
2:00 pm - 3:30 pm	Poster Prep
4:30 pm - 5:00 pm	Lab Clean-up

Computers must be returned to stockroom by 4:30pm

updated 6/15/09