Chemistry 340 Quiz 13 |
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1-2. Answer with (C) cyt c (Fe2+); (D) cyt c (3+); (F) FADH2; (G) FAD; (H) H2; (I) O2;
(N) NADH; (O) NAD+; (Q) QH2; (R) Q; (S) succinate; (T) fumarate.
The whole reaction catalyzed by Complex I is N + R → O + Q .
You do not need to balance the equation. *
3-4. Write the total number of H+ moved N → P by the specified complex only.
0 Complex II oxidizes 4 succinate. |
4 Complex IV oxidizes 4 cyt c (Fe2+). |
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5-7. Answer with the appropriate part or conformation of ATP synthase: Fo or F1; subunits a, β, or c; conformations L, O, or T. Use a choice only once. |
8-10. This is a substrate in the citric acid cycle. Draw the product.
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β the subunit with the active site |
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O the conformation that releases ATP |
Enzyme name: succinate dehydrogenase |
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F1 the part of ATP synthase that makes ATP |
Coenzyme produced: FADH2 or QH2 |
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1-2. Answer with (C) cyt c (Fe2+); (D) cyt c (Fe3+); (F) FADH2; (G) FAD; (H) H2; (I) O2;
(N) NADH; (O) NAD+; (Q) QH2; (R) Q; (S) succinate; (T) fumarate.
The whole reaction catalyzed by Complex II is S + R → T + Q .
You do not need to balance the equation. *
3-4. Write the total number of H+ moved N → P by the specified complex only.
Complex I oxidizes 4 NADH. |
Complex III completely oxidizes 2 QH2. |
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5-7. Answer with the appropriate part or conformation of ATP synthase: Fo or F1; subunits a, β, or c; conformations L, O, or T. Use a choice only once. |
8-10. This is a substrate in the citric acid cycle. Draw the product.
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β There are 3 subunits/ATP synthase |
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a the subunit with a half channel open to the P side |
Enzyme name: isocitrate dehydrogenase |
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T the conformation that forms the ADP-P bond |
Coenzyme produced: NADH |
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1-2. Answer with (C) cyt c (Fe2+); (D) cyt c (Fe3+); (F) FADH2; (G) FAD; (H) H2; (I) O2;
(N) NADH; (O) NAD+; (Q) QH2; (R) Q; (S) succinate; (T) fumarate.
The whole reaction catalyzed by Complex III is Q + D → R + C .
You do not need to balance the equation. *
3-4. Write the total number of H+ moved N → P by the specified complex only.
20 Complex I oxidizes 5 NADH. |
6 Complex IV oxidizes 6 cyt c (Fe2+). |
5-7. Answer with the appropriate part or conformation of ATP synthase: Fo or F1; subunits a, β, or c; conformations L, O, or T. Use a choice only once. |
8-10. This is a substrate in the citric acid cycle. Draw the product.
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c the subunit with Asp |
Enzyme name: malate dehydrogenase |
L the conformation that binds ADP |
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Fo part of ATP synthase that transports |
Coenzyme produced: NADH |
1-2. Answer with (C) cyt c (Fe2+); (D) cyt c (Fe3+); (F) FADH2; (G) FAD; (H) H2; (I) O2;
(N) NADH; (O) NAD+; (Q) QH2; (R) Q; (S) succinate; (T) fumarate.
The whole reaction catalyzed by Complex IV is C + I → D + H .
You do not need to balance the equation. *
3-4. Write the total number of H+ moved N → P by the specified complex only.
0 Complex II oxidizes 4 succinate. |
12 Complex III completely oxidizes 3 QH2. |
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5-7. Answer with the appropriate part or conformation of ATP synthase: Fo or F1; subunits a, β, or c; conformations L, O, or T. Use a choice only once. |
8-10. This is a substrate in the citric acid cycle. Draw the product.
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T the conformation that forms the ADP-P bond |
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c 1 ATP synthase has ∼ 10 subunits |
Enzyme name: succinyl-CoA synthetase |
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F1 the part of ATP synthase that is in the matrix |
Coenzyme produced: GTP (CoA-SH accepted) |
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*Comment on grading 1-2: 1 point was for choosing the correct substrates and products; 1 point was for combining them in a redox reaction that was spontaneous. A quiz that says "-0.5 choice" indicates that the complex actually has a different substrate. A quiz that says "-1 redox" indicates that (usually) two reduced substrates went to two oxidized products, which is not a redox reaction.
