Exercise on Tertiary and Quaternary Protein Structure |
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Tertiary and Quaternary Protein Structure
1. Go to the Protein Data Bank web site.
2. Search for two of the following proteins.
For each, click on Sequence Details and use the information to fill in the table.
protein |
kinase |
dehydrogenase |
trypsin |
synthetase |
phosphatase |
example |
1HKC |
2DLD |
1MTS |
1PFY |
1BIF |
functional class |
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number of chains |
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number of residues |
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number of domains |
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number of disulfide bonds |
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number of α helices; residues |
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β strands; residues |
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more α or β? |
Answers:
characteristic |
kinase |
dehydrogenase |
trypsin |
synthetase |
phosphatase |
|
example |
1HKC |
2DLD |
1MTS |
1PFY |
1BIF |
|
functional class |
trans-ferase |
oxidoreductase |
serine protease |
ligase |
hydrolase |
|
number of chains; identical? |
1 |
2 identical |
1 |
1 |
1 |
|
number of domains |
4 |
2 |
1 |
3 |
2 |
|
number of residues |
917 |
337 |
245 |
551 |
469 |
|
number of disulfide bonds |
0 |
0 |
7 |
0 |
0 |
|
number of α helices |
36; 385 |
16; 130 |
2; 10 |
26; 275 |
19; 187 |
|
β strands; residues |
31; 169 |
22; 55 |
13; 70 |
28; 90 |
16; 67 |
|
more α or β? |
α |
α |
β |
α |
α |
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